Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE $message = SendPassword($link, $user, $login); Senior Researcher, EURAC Research, "I work for a fast-paced drug discovery CRO where our clients expect high-quality data with rapid turnarounds. Bio-Techne uses cookies to provide you with a great website experience. How can I find a Simple Western-validated antibody? Copyright 2023 Bio-Techne. Novus Antibodies Validated for Simple Western. | How Can Simple Western Help You? Simple Western instrument software Compass for Simple Western analyzes Simple Western Size or Charge assay data and processes the results for you. Senior Research Scientist, TCG Lifesciences, "Jess allows me to reduce the preparation time, save on reagents, samples, and antibodies, while allowing easier optimization of target proteins/antibodies. ProteinSimple branded instruments are available to quote. With Wes, we can do multiple assays with one sample collection. This database is intended to provide assay general development guidance in identifying and selecting antibodies to test. Novus Antibodies Validated for Simple Western, Simple Western Frequently Asked Questions (FAQ). Protein characterization should be easy and with Simple Western, it can be. ", Irene Tobias, Ph.D., ACD branded products will be available on biotechne.com in the near future. I really like this system over traditional Western. A validated workflow for protein analysis in extracellular vesicle samples from the leaders in exosome isolation and high sensitivity western blotting solutions. View all citations for the Simple Western platform. ", Dr. Michael Harte, Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE More Than Just a Western Blot Alternative. ", Joseph Klebba, Ph.D., View all citations for the Simple Western platform. Assistant Professor, Department of Pharmacology and Toxicology, University of Texas Medical Branch, "We collect samples from laser microdissection and were using our entire sample on just one regular Western blot. Which Brands are Currently Available on biotechne.com? RePlex on Jess and Abby lowers the cost per result compared to traditional Western blotting protocols. Pipette your samples into her plate and let her do the rest! Translating Traditional Western Blots to Simple Western is Fast and Easy, Advancing Cell and Gene Therapies with Jess, Restoring Autophagy, Reversing Disease with Jess, Utilize Simple Westerns for Protein Analysis of Osteoarthritis Patient Samples, Utilizing Simple Western to Understand Neuronal Markers in Schizophrenia, Noelle Changes Her Brain Receptor Research Game with Wes, Mary Ups Her Data Point Count on Laser Capture Microdissection Samples with Wes, Miranda Sets a New Pace for Alzheimer's Research with Wes, Brian Cuts Time to Results and Solves His Membrane Protein Challenges with Wes, Jamie Keeps Things Greener with Simple Western, Mary Makes More Links Between Obesity and Cancer with NanoPro 1000, Emma Gets to Better Outcomes for Osteoarthritis Patients with Wes, Far Western Blot With Simple Western To Detect Protein Phosphorylation, Jane Gets Fast Answers for Cancer Research with Wes, Detecting Low Abundance Proteins in Precious Pediatric Cancer Samples with Wes, Lower Limit of Detection Advances Understanding of Metabolic Signaling in Human Muscle, Quantifying Protein Tyrosine Phosphatase in Platelets, Wes Zips Through Pharmacodynamic Biomarker Discovery Projects at the University of Glasgow, Wes Validates the Potential of RNA-based Metabolic Therapies At MiNA Therapeutics, Simple Western and the Race to Develop a Vaccine for the COVID-19 Coronavirus, Rebel without a Cause - Searching for the Origins of Parkinson's Disease with Wes, PROTAC Studies Take Off in Drug Discovery, and Jess is on Board, Jess Breaks New Quantitative Ground in New Chemical Entity Screening at TCG Lifesciences, Conducting Novel Therapeutics Research of Human Gliomas with Jess, Unraveling Neurodegenerative Diseases with Jess, Detect Phosphorylation of Proteins with RePlex, Elena Unlocks Immune Cell Potential with Simple Western and CRISPR/Cas9, Muhammad Uses Simple Western to Chart Stem Cell Signaling Pathways, Investigating Resolution of Inflammation with Jess, Delving into the Complexities of Endometrial Receptivity with Simple Western, Simple Western Individual Charge Reagents, Download PL9-0034 RevF SW Buffer Compatibility Table, Download 043-125 RevD Jess Wes Abby-installation guide, Molecular weight (MW) ladders range from 2-440 kDa, Wes - Automated Western Blots with Simple Western | ProteinSimple. Cardiovascular Research Technician, Department of Pediatrics, University of Alberta Faculty of Medicine & Dentistry, "The Simple Western Charge assay, a capillary isoelectric focusing immunoassay, exceeded the reliability of 2D Western blots for resolving recombinant PKG-I and PKG-I and uses 100,000X less sample quantity, making it well-suited for clinical disease proteomics because protein isoforms and post-translational modifications can be detected in precious tissue biopsies. to test. Research Fellow, Brain Research Institute Monash Sunway (BRIMS), "It saves so much time! All Rights Reserved. Custom Targeted Protein Degradation Services, Certificate of Analysis (COA) Finder Tool. 2023 ProteinSimple. Minneapolis, MN 55413 Novus Biologicals branded products are not currently available on biotechne.com and can be found at novusbio.com. ACD branded products will be available on biotechne.com in the near future. Copyright 2023 Bio-Techne. This database is ", Dr. Michael Harte, Toll-free 800 343 7475 Jesss ability to detect multiple proteins per sample further saves time compared to conventional Western blot. Senior Research Scientist, Aurelia Bioscience, Biocity, Nottingham, UK, "The quantitative assays and short run times on Jess enable us to generate data faster and be more confident in our results. A specific band was detected for cIAP1/HIAP2 at approximately 66 kDa (as indicated) in the parental HeLa cell line but is not detectable in knockout HeLa cell line. $pageheader = new BannerMenus_cellbio($link, $DB_USER_TABLE, $path); Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments. global $HTTP_GET_VARS; Simple Western is your protein characterization problem-solver. A specific band was detected for cIAP1/HIAP2 at approximately 72 kDa. And obviously I like getting results the same day rather than having to wait two days", Emma Wilson (ne Humphrey), Ph.D., Postdoctoral Fellow, University of Texas Health Science Center, "We had been trying to study receptor proteins with traditional Westerns but it was frustratingwe just could not get it. // The code continues at the bottom of the page Nuffield Department of Medicine, Target Discovery Institute, "Protein analysis using [Simple Western] can be performed faster, eliminating tedious work and saving time. Set up, leave and remotely review your data in just 3 hours! DisplayLogin(0, $path, $status, $message, $headfoot, $bannermenus, $userid, $page, $docid, $login); The results are much better too, given that it removes the blotting step all together. $page = GetParamDefault("page", $defpage); Check out other antibodies that have been screened and tested with Simple Western Charge- and Size-based assays! SIMPLE Simple Western Systems Simple Western Antibody Database Simple Western Antibody Submission AIF VENDOR: R&D Systems CAT #: AF1457 purified Polyclonal Antibody (Catalog # AF1457 experiment was conducted under reducing conditions and using This antibody is certified for Gel-Free, Blot Free, Hands Free Simple Western Systems. Associate Professor, Department of Genetics and Genome Sciences, UConn Health, "Weve been able to increase productivity dramatically with Wes. Abby allows you to detect total / phospho targets within the same capillary using RePlex. antibodies for use on Milo, check out our tech note. What tissue homogenization buffer can be used for Simple Western. "Using Simple Western has been extremely rewarding, especially due to how easy it was to transfer our western blotting assays to a fully automated format on Simple Western and how well CST antibodies perform on the platform. This technology has made it possible for small research labs to compete with the pace that research is conducted in large labs or companies. Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE In exchange for 30 minutes of easy experimental setup, you get the results of 24 fully-analyzed samples after 3 hours of hands-free run time. $id = $_SESSION["userid"]; This database is intended to provide general assay Eliminates the variability of traditional Western blotting protocols like the gel-to-membrane transfer to get accurate measurements of target proteins. Lecturer in Molecular Biology, Institute of Medicine, University of Chester, "The no-gel, no-transfer, no-membrane features are beneficial for the reproducibility of the assay. Her picogram sensitivity and total protein detection, combined with fully analyzed results in 3 5 hours, ensure that you'll never want to touch a traditional Western blot again! $url = $page; // May already be logged in: No problem. Useas little as 3 L of starting material. Our Wes runs are completed in less than 4 hours, as opposed to 4 days with traditional methods. Faculty Fellow, Wolfson Childhood Cancer Research Centre, Northern Institute for Cancer Research, Newcastle University, "Wes allows us to measure cell signaling proteins from a single muscle fiber segment by lowering our limit of detection nearly ~20-fold from Western blotting. ProteinSimple branded instruments are available to quote. antibodies that have been screened and tested in Simple Western Size- and Senior Researcher, EURAC Research, "I work for a fast-paced drug discovery CRO where our clients expect high-quality data with rapid turnarounds. $title = 'Simple Western antibody database :: ProteinSimple'; } else if ($action == "sendpass") { } Research Fellow, Brain Research Institute Monash Sunway (BRIMS), "It saves so much time! Copyright 2023 Bio-Techne. if ($_SESSION["userid"]) { Wes has been discontinued as of July 30th, 2021. ", Jon Voutila, Ph.D., Please visit our new Simple Western Antibody Database on Bio-Techne.com for the latest antibody information. How's that for reproducibility? ", Miranda Orr, Ph.D., With Wes, we can do multiple assays with one sample collection. $description = ''; FAX 612 656 4400. Toll-free 800 343 7475 Compass for Simple Western Software's export feature makes this as easy as a click of a button. RePlex with Abby enables you to run two immunoassays within the same capillary. The Simple Western antibody database has moved! Associate Professor, Department of Physiology and Cell Biology, University of Nevada School of Medicine, "The traditional blotting method can use up to 300 pieces of plastic and [Simple Western] uses two. global $HTTP_GET_VARS; I really like this system over traditional Western. Over 1,300 Novus Biologicals and R&D Systems antibodies have been certified on ProteinSimple's Simple Western platforms. He found that traditional Western blot uses up to 300 pieces of plastic and Wes uses two. ProteinSimple branded instruments are available to quote. if ($docid != 0) { Novus Biologicals branded products are not currently available on biotechne.com and can be found at novusbio.com. It takes away so much of the pipetting and manipulation error. The throughput and system automation is a definite advantage. Assistant Professor, Womens and Childrens Health, Karolinska Institute, Stockholm, Sweden. Save time and money and boost your productivity with our automated Western blot processors, total protein detection to accurately normalize protein expression data, Size-based automated capillary Western blot assays, Size and charge-based automated capillary Western blot assays, Charge-based automated capillary Western blot assays. Having problems detecting your protein of interest? With this leap in sensitivity, Stellar fluorescence detection rivals the widely recognized sensitivity of chemiluminescence detection and demolishes the fluorescence detection of the closest competing traditional Western blot imaging instrument, which requires at least 50 pg. Data analysis with Compasss user-friendly software is quick and easy. A validated workflow for protein analysis in extracellular vesicle samples from the leaders in exosome isolation and high sensitivity Western blotting solutions. Rolling over a band will reveal its protein-specific information. It would take about a week to run 40 proteins using Western blotting. Tired of being handcuffed to your bench for hours tending to your gels and blots? $title = 'Simple Western antibody database :: ProteinSimple'; Simple Western8x25Simple Western Antibody Submission Promotion . When Access Control is enabled and the new Authorization Server has been installed: Which Brands are Currently Available on biotechne.com? Senior Research Scientist, Aurelia Bioscience, Biocity, Nottingham, UK, "The quantitative assays and short run times on Jess enable us to generate data faster and be more confident in our results. Want to share your results and transfer your assay? RePlex efficiently removes primary antibodies and secondary antibodies between probing cycles because samples are covalently immobilized, allowing for excellent reproducibility across cycles. Which Brands are Currently Available on biotechne.com? DisplayLogin(0, $path, $status, $message, $headfoot, $bannermenus, $userid, $page, $docid, $login); $pass = strip_tags($HTTP_POST_VARS["pass"]); Whether you want to do absolute or relative quantitation of your target, you need to reliably be able to detect signal saturation and work within the linear dynamic range of your assay. $action = strip_tags($HTTP_GET_VARS["action"]); to probe scWest chips. ProteinSimple will continue service and support of the Wes platform through July 30th, 2026. exhaustive. This allows us to use more advanced statistical techniques such as principal component analysis and hierarchal clustering to recognize key correlations in our research with ease", John K. Blackwood PhD, ACD branded products will be available on biotechne.com in the near future. Early Development Scientist, Oxford Biomedica, "Jess allows us to immediately identify potential autophagy biomarkers as they are upregulated or downregulated in response to treatment. $description = ''; With a processing time of 3 hours for 25 samples or 96 samples overnight, Simple Western dramatically speeds up time to results compared to traditional Western blotting protocols. Nuffield Department of Medicine, Target Discovery Institute, "Protein analysis using [Simple Western] can be performed faster, eliminating tedious work and saving time. For more information on how to select antibodies for use on Milo, check out our tech note. Laboratory Coordinator, Department of Internal Medicine, East Tennessee State University, "The rapid, reproducible results on small amounts of tissue/cell lysates have allowed me to generate a more thorough data set on additional proteins, samples, conditions and brain regions all in the same amount, or even less time, as traditional Western blot. You just load your samples and wait for results, and in the meantime, you can dedicate yourself to other activities. By continuing to use this website you acknowledge this and agree to our cookie policy. page_head_start($title, $description); It takes away so much of the pipetting and manipulation error. Simple This experiment was performed under reducing conditions using the 12-230 kDa separation system. Associate Professor, Department of Genetics and Genome Sciences, UConn Health, "Weve been able to increase productivity dramatically with Wes. And obviously I like getting results the same day rather than having to wait two days", Emma Wilson (ne Humphrey), Ph.D., With channel-basedmultiplexing, you can detect multiple target proteins per cycle. Data can be displayed in a lane view, graph view, or as a digital capillary image. FAX 612 656 4400. Professor and Chair, University of Birmingham, UK, "I was able to screen various antibodies in a single run within half a day. $status = strip_tags($HTTP_GET_VARS["status"]); And it's got all the 21 CFR Part 11 compliance tools you need with the new Compass Authorization Server v3.0.0 (more details below). // Custom page code My antibody is validated for Western blot. Scientist, Vividion Therapeutics, "I used Simple Western because the protein samples I had were small. With Simple Western, researchers save samples, reagents, wash buffer, time, and labor! USA, TEL 612 379 2956 FAX 612 656 4400. the antibodies that have been validated on Milo to date. ProteinSimple will continue service and support of the Wes platform through July 30th, 2026. Imagine all the data you can get from your sample! No problem! Senior Product Scientist, Cell Signaling Technology (CST), "Simple Western allows us to run three-hour assays, which would usually take us two days with traditional Western blots. Scientist, Vividion Therapeutics, "I used Simple Western because the protein samples I had were small. Note: in cases where ", Melyssa Bratton, Ph.D., // End custom page code Postdoctoral Research Scientist, Institute of Cancer Sciences, University of Glasgow, "I can run immunoassays on Wes for an entire animal experiment in a single day. Compare the expression of proteins of interest to total protein. Simple Western separates proteins by size, and precisely manages immunoprobing, incubations, washes and even detection steps by removing the manual, error-prone steps in standard Western blots. The database is intended to be used for guidance in target identification, but results can vary depending on the systems . The Simple Western antibody database is a user-interactive listing of antibodies that have been screened and tested in Simple Western Charge- and Size-based assays. Screen for processing-related impurities and host-cell related contaminants. Postdoc, DIOSynVax, Department of Veterinary Medicine, Cambridge, United Kingdom, "Its had a significant impact on our research we get double the amount of data in a third of the time. FAX 612 656 4400. Approved submissions receive: Free Separation Module (8x25 capillary cartridges) for an antibody against a new target not currently in the Simple Western database. Set up, leave and remotely review your data in just 3 hours! Moving away from the tedious manual western blotting process, which can take up to two days, and introducing automation will speed up time to data and provide increased sensitivity and accuracy. Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows. performing antibody is indicated by *. Is your current method unable to handle the throughput you need? Abby solves both problems with her chemiluminescent detection. |How Can Simple Western Help You? } else { HRP-conjugated Anti-Mouse IgG Secondary Antibody. ValidateLogin('none,sales,antibodies', "index.html", " to view and submit antibodies"); ", Yotis Senis, Ph.D., All Rights Reserved. By continuing to use this website you acknowledge this and agree to our cookie policy. ", Dr. Mattia Volta, Featured inwell over 1500publicationsand rapidly growing, Simple Western is aproven technologyin all types ofresearch applications, going far beyond what can be done with traditional Western blotting. ", Dr. Mattia Volta, R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through biotechne.com. ", Elena Navarro-Guerrero, Ph.D., Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows. Join us in the Bio-Techne Academy, our first workshop begins on January 26th, 2023! Simple Western Antibody Database on Bio-Techne.com, Simple Western Antibody Submission Promotion, Lovo (left) and HeLa (right) lysate 0.5 mg/ml. CleanHTTPVars(); Toll-free 800 343 7475 Jesss ability to detect multiple proteins per sample further saves time compared to conventional Western blot. High-throughput pharmacokinetic analysis with quantitative, high-sensitivity assays directly in complex lysates and tissue homogenates. Minneapolis, MN 55413 ", Nicolas G. Bazan, MD, PhD, Searching for key inflammation molecules", Kirstine Nolling Jensen, PhD Student, Proteins are displayed as bands in a lane view similar to what you would see in a traditional Western. ", Chris LaBreck, Ph.D., Milo is an open platform so you can use any Western-validated antibody Is your sample too precious or too expensive? ", Dr. Matteo Ferrari, Senior Product Scientist, Cell Signaling Technology (CST), "Simple Western allows us to run three-hour assays, which would usually take us two days with traditional Western blots. Just load your samples, press start, and come back to analyzed results. Welcome to the Simple Western antibody database, which provides information on the growing number of antibodies that have been screened and tested by Simple Western Charge- and Size-based assay users in their analysis. $section = 'learn'; Fast Time to Results. Submit your antibody validation data and get a free Separation or Detection Module! // Custom page code ", Mary G. Johlfs, M.S., Reproducible and Quantitative. Postdoctoral Researcher, CSU Fullerton, "[Wes] Provides quantitative data, saves time and uses far less sample. Bio-Techne uses cookies to provide you with a great website experience. Searching for key inflammation molecules", Kirstine Nolling Jensen, PhD Student, Some features highlights include: The Compass Access Control feature has been updated! This is something I wouldnt even try with traditional Western blots. $id = strip_tags($HTTP_GET_VARS["id"]); Researchers get accurate measurements of target proteins withTotal Protein Assaysand built-inTotal Protein Normalization. The low protein concentrations required by Wes makes it easier to save precious samples ensuring protein detection. Early Development Scientist, Oxford Biomedica, "Jess allows us to immediately identify potential autophagy biomarkers as they are upregulated or downregulated in response to treatment. Senior Research Scientist, TCG Lifesciences, "Jess allows me to reduce the preparation time, save on reagents, samples, and antibodies, while allowing easier optimization of target proteins/antibodies. Traditional Westerns are as much an art form as they are a technical tool. GoToPage("index.html?status=$status"); Minneapolis, MN 55413 See full datasheet. Mouse Anti-Human cIAP1/HIAP2 Monoclonal Antibody (Catalog # MAB818) was used at 5 g/mL. performance of any listed antibody will need to be optimized for the With Wes, you can achieve intra-assay CV less than 15%. WES HAS BEEN DISCONTINUED AS OF JULY 30TH, 2021. Find answers to frequently asked questions (FAQ) about Simple Western from ProteinSimple, a Bio-Techne brand, including: Accelerate your assay development on Simple Western by learning from the experts! All Rights Reserved. Are your assays lacking the reproducibility and reliability that you need to be confident in your research? Chemiluminescent detection:Are you working with low abundance proteins or have little sample to spare for analysis? Is yoursample too precious or too expensive? Improve reproducibility, quantitation, and time to market for your vaccine candidates. This database is intended to provide general assay development guidance for identifying and selecting antibodies to test. Boost your screening studies using high throughput & hands-free Simple Western systems for quantitative analysis. intended to provide general guidance in identifying and selecting antibodies Cardiovascular Research Technician, Department of Pediatrics, University of Alberta Faculty of Medicine & Dentistry, "The Simple Western Charge assay, a capillary isoelectric focusing immunoassay, exceeded the reliability of 2D Western blots for resolving recombinant PKG-I and PKG-I and uses 100,000X less sample quantity, making it well-suited for clinical disease proteomics because protein isoforms and post-translational modifications can be detected in precious tissue biopsies. Western Antibody Submission Promotion. Copyright 2023 Bio-Techne. Custom Targeted Protein Degradation Services, Certificate of Analysis (COA) Finder Tool, Stellar High Sensitivity NIR/IR Detection. With HDR detection profile and analysis Wes gets you up to 6 logs of dynamic range. Enjoy picogram-level sensitivity that enables you to get the most data out of your samples. ", Dr. Mario Rothbauer, // The output below is part of DisplayLogin() Submit new antibody validation Postdoctoral Researcher, CSU Fullerton, "[Wes] Provides quantitative data, saves time and uses far less sample. Generate up to 8 data points with just 5 L. ACD branded products will be available on biotechne.com in the near future. specific sample type being studied. By eliminating the variability of traditional Western blotting protocols like the gel-to-membrane transfer, Simple Westernassays are highly reproducible and fully quantitative. Do you need a method that is quantitative? Join us at the Bio-Techne Academy - our first workshop begins on January 26th, 2023! Are you struggling to keep up with your sample workload because lab availability is limited? Submit your antibody validation data and get a free Separation or Detection Module! How can I find a Simple Western-validated antibody? /* Load any additional CSS and JS files here */ ", Noelle Anastasio, Ph.D., /* Load any additional CSS and JS files here */ Simple Western is your protein characterization problem-solver. $message = strip_tags($HTTP_GET_VARS["message"]); Assistant Professor, Department of Pharmacology and Toxicology, University of Texas Medical Branch, "We collect samples from laser microdissection and were using our entire sample on just one regular Western blot. USA, TEL 612 379 2956 The performance of any antibody will need to be optimized for the specific sample type being studied. A few months with Wes and we've already had way more success than we had in three years. See what other researchers are saying about Simple Western! global $HTTP_POST_VARS; ", Joseph Klebba, Ph.D., 2023 Novus Biologicals, All Rights Reserved. Chemiluminescent detection gives you picogram-level sensitivity, letting you maximize the data you get from your sample. The throughput and system automation is a definite advantage. All Rights Reserved. ", Muhumad Noor Alfarizal Kamarudin, Set up, leave and remotely review your data in just 3 hours!